The results indicated that the predicted protein molecular weights of L-PRLR and S-PRLR are 65235.36 KD and 33847.48 KD, respectively, with isoelectric points of 5.12 and 8.34, respectively. The physical and chemical properties, the similarity of the nucleotides L-PRLR and S-PRLR genes and the secondary and tertiary structure of the L-PRLR and S-PRLR proteins of sheep were analyzed. Samples of uterus, mammary gland, ovary, spleen and lymph tissue were collected from each ewe to determine the mRNA expression of long PRLR ( L-PRLR) and short PRLR ( S-PRLR) by RT-qPCR. The remaining five ewes were slaughtered on the 5th day after weaning. Five ewes were chosen randomly and slaughtered at mid-lactation (35 days after lambing). Ten lactating crossbred ewes (Dorper×Hu sheep) with twin lambs were used in this study. The objective of this study was to analyze the gene sequence and mRNA expression of PRLR isoforms in the uterus, mammary gland, ovary, spleen and lymph tissue of ewes during the lactation and post-weaning periods. PeerJ 9: e11868 įew studies on mRNA expression of the prolactin receptor ( PRLR) isoforms in different tissues of sheep were reported. Sequence analysis and mRNA expression of prolactin receptor gene isoforms in different tissues of sheep during lactation and the post-weaning period. Cite this article Yang R, Duan C, Guo Y, Ma Y, Niu N, Zhang Y, Liu Y. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Licence This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. PeerJ - General bio (stats, legal, policy, edu)Ģ College of Life Sciences, Hebei Agricultural University, Baoding, Hebei, China DOI 10.7717/peerj.11868 Published Accepted Received Academic Editor Mudasir Ahmad Syed Subject Areas Agricultural Science, Biochemistry, Genetics, Molecular Biology, Veterinary Medicine Keywords Sheep, Prolactin receptor, Lactation, Post-weaning Copyright © 2021 Yang et al.PeerJ - Biology & Life science articles.Table of Contents - current and archives.PeerJ (Life, Biological, Environmental and Health Sciences).Biochemistry, Biophysics and Molecular Biology.